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KMID : 0895820070170010067
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Journal of Oriental Rehabilitation Medicine
2007 Volume.17 No. 1 p.67 ~ p.74
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Effect of Nardostachytis Rhizoma on Induction of Differentiation in HL-60 Cells
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Yun Sang-Hak
Park Jong-Ha
Kim Kyeong-Su
Oh Won-Kyo
Yeom Seung-Ryong
Kwon Young-Dal
Song Yung-Sun
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Abstract
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Objectives: To investigate PKC/MAPKs signaling pathways on the human promyelocytic leukemia HL-60 cell differentiation induced by Nardostachytis Rhizoma (N. Rhizoma).
Methods: Differentiation of HL-60 cells was determined through expression of CD11b and CD14 surface antigens using flow cytometry and nitroblue tetrazolium (NBT) assay. PKC activity was measured by using a PKC Kinase Activity Assay kit (Non-Radioactive). Activation of ERK and protein levels of PKC isoforms were determined by Western blot analysis.
Results: Activation of extracellular signal-regulated kinase (ERK) increased time-dependently in differentiation of HL-60 cells induced by N. Rhizoma. Inhibitor of ERK (PD98059) significantly reduced NBT reduction activity induced by N. Rhizoma in HL-60 cells. In addition, N. Rhizoma increased protein level of PKC α, PKC βI and PKC βII isoforms, but the protein level of PKC γ was constant. PKC inhibitors (GF 109203X, Chelerythrine and H-7) inhibited HL-60 cell differentiation and the ERK activation induced by N. Rhizoma.
Conclusions: N. Rhizoma induces granulocytic differentiation via a PKC/ERK signaling pathway in HL-60 cells.
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KEYWORD
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Nardostachytis Rhizoma, HL-60 cell, Differentiation
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